Amphomycin acylase.
نویسندگان
چکیده
منابع مشابه
acylase from Providencia rettgeri
Two isoforms of the heterodimeric enzyme penicillin G acylase (EC 3.5.1.1 I ) from Providencia rettgeri ATCC 31052 (strain Brol) were purified to near homogeneity. The isoforms exhibited comparable enzymatic a tivities but differed slightly in the molecular weight and PI of their respective a-subunit. The origin of this difference was traced to the partial conversion of the N-terminal Gln of th...
متن کاملProduction of Penicillin Acylase.
The production of penicillin acylase by Escherichia coli Ny.I/3-67 has been increased by phenylacetic acid and phenoxyacetic acid, which themselves strongly inhibit the function of this specific enzyme. Other carbonic acids also increased penicillin acylase production, but to a lesser degree; they also weakly inhibited enzyme function. The production of this enzyme was effectively repressed wit...
متن کاملOn the "minor basic amino acid residues" of amphomycin.
Sir: In a recent communication1* from this laboratory it was reported that in addition to L-threoand D-erythro-a, /?-diaminobutyric acids some yet unidentified basic amino acids also occur in the acid hydrolysates of amphomycin2). These minor constituents were provisionally designated according to their relative elution times (in minutes counting from the ammonia peak) on the short column of th...
متن کاملAmphomycin inhibits mannosylphosphoryldolichol synthesis by forming a complex with dolichylmonophosphate.
The inhibitory effect of the lipopeptide antibiotic amphomycin on the mechanism of mannosylphosphoryldolichol biosynthesis by calf brain rough endoplasmic reticulum membranes has been studied extensively. Calf brain rough endoplasmic reticulum membranes when incubated with varying concentrations of GDP-mannose in the presence and absence of amphomycin showed no significant difference in apparen...
متن کاملProtein Engineering of Penicillin Acylase
Penicillin acylases (PA) are widely used for the production of semi-synthetic β-lactam antibiotics and chiral compounds. In this review, the latest achievements in the production of recombinant enzymes are discussed, as well as the results of PA type G protein engineering.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1978
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.31.373